JoVE Journal
Genetics
Genetics
Se requiere una suscripción a JoVE para ver este contenido.
Capítulos
Resumen
The identification of physical interactions between genes and regulatory elements is challenging but has been facilitated by chromosome conformation capture methods. This modification to the 4C-seq protocol mitigates PCR bias by minimizing over-amplification of PCR templates and maximizes the mappability of reads by incorporating an addition restriction enzyme digest step.