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Developmental Biology

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JoVE Journal Developmental Biology
Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Journal (Developmental Biology)
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
Journal (Developmental Biology)
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
 

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Article DOI: 10.3791/58216-v • 07:48 min • September 6th, 2018
September 6th, 2018 •
1,2,3, 1,2,3, 1,2,3, 4, 5, 1,2
1U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), 2U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), 3I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), 4Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), 5Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

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Resumen
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We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.

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Engineering Transplantation Retinal Pigment Epithelium Tissue Human Embryonic Stem Cells Bio-engineered Tissue In Vivo Efficacy Eye Therapy Contouring Eye Implantation Human Amniotic Membrane Nylon Scaffolds PBS Thermolysin Solution Culture Dish Culture Insert
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