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Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

Article DOI: 10.3791/58229-v • 06:23 min • October 30th, 2018
October 30th, 2018 •
1
1Division of Neuromedical Science, Institute of Natural Medicine, University of Toyama

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Here a protocol to investigate the early effects of amyloid-β (Aβ) in the brain is presented. This shows that Aβ induces clathrin-mediated endocytosis and collapse of axonal growth cones. The protocol is useful in studying early effects of Aβ on axonal growth cones and may facilitate prevention of Alzheimer's disease.

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Axonal Growth Cone Collapse Early Amyloid β Effects Alzheimer's Disease Research Visualizing Analyzing Micro-scissors Mince Cerebral Cortices Embryonic Day-14 Mice Neuron Culture Medium Antibiotics Centrifuge Trypsin Incubate Neutralize DNAse Soybean Trypsin Inhibitor Solution Medium A Triturate Polished Pasteur Pipette Debris Filter Cell Count
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