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Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates

Article DOI: 10.3791/58359-v • 08:33 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1
1Physics Department, Bar-Ilan University

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A HMCA-based imaging plate is presented for invasion assay performance. This plate facilitates the formation of three-dimensional (3D) tumor spheroids and the measurement of cancer cell invasion into the extracellular matrix (ECM). The invasion assay quantification is achieved by semi-automatic analysis.

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Cancer Cell Invasion Anti-metastatic Drug Screening Hydrogel Micro-chamber Array Tumor Spheroid Reproducible Biomimetic 3D Cell-based System Invasion Capacity Analysis Uniform Spheroids Focal Plane Extracellular Matrix Morphological Characterization Fluorescent Staining Time-efficient Manner Hydrogel Micro-chamber Array Plates Polydimethylsiloxane (PDMS) Low-melting Agarose
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