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How to Stabilize Protein
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How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Article DOI: 10.3791/58666-v • 07:22 min • February 11th, 2019
February 11th, 2019 •
1, 1, 2, 1,2
1Department of Biosciences, Durham University, 2Department of Chemistry, Durham University

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A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry. Buffer systems, salts and additives, together comprising three unique stability screens, are assayed with proteins to identify suitable buffers for functional and structural studies.

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Stabilize Protein Stability Screens Thermal Shift Assay Nano Differential Scanning Fluorimetry Protein Chemistry Drug Discovery Thermostability Mutations Ligands Advantages Fast Standard Laboratory Medium To High Throughput Protein Sample Multichannel Pipette Buffer System SYPRO Orange Dye 96 Well Plate Centrifuge Storage TSA Experiment RTPCR System
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