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Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Article DOI: 10.3791/59127-v • 08:11 min • January 31st, 2019
January 31st, 2019 •
1, 1, 1, 1, 1,2, 1
1Frankfurt Initiative for Regenerative Medicine, Johann Wolfgang Goethe-University, 2J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida

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Here we present a protocol for the construction of a cell culture chamber designed to expose cells to various types of electrical stimulation, and its use in treating mesenchymal stem cells to enhance osteogenic differentiation.

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Electrical Stimulation Chamber Osteogenic Differentiation Mesenchymal Stem Cells Bone Tissue Engineering Cell Types Electro-sensitive Cell Behaviors Platinum Wire Silver-coated Wire Six Well Plate Lid Super-conductive Glue
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