Híbrido claro/azul electroforesis nativa para la separación y análisis de Supercomplejos de cadenas respiratorias mitocondriales
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11:25 min
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May 19, 2019
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Aquí presentamos un protocolo para extraer, resolver e identificar los supercomplejos mitocondriales que minimizan la exposición a los detergentes y al azul de Coomassie. Este protocolo ofrece un equilibrio óptimo entre la resolución y la preservación de las actividades enzimáticas, al tiempo que minimiza el riesgo de pérdida de interacciones entre proteínas y proteínas del lábil.
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Cite this Article
Cuillerier, A., Burelle, Y. Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes. J. Vis. Exp. (147), e59294, doi:10.3791/59294 (2019).
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