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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Article DOI: 10.3791/59382-v • 10:10 min • March 31st, 2019
March 31st, 2019 •
1, 2, 2, 1, 1, 3, 1
1Department of Pediatrics, Pennsylvania State University College of Medicine, 2Department of Physiological Sciences, University of Florida, 3Department of Anatomy and Cell Biology, University of Florida

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A CRISPR/sgRNA library has been applied to interrogating protein-coding genes.However, the feasibility of a sgRNA library to uncover the function of a CTCF boundary in gene regulation remains unexplored. Here, we describe a HOX loci specific sgRNA library to elucidate the function of CTCF boundaries in HOX loci.

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HOX Loci CRISPR SgRNA Library Screening CTCF Chromatin Boundaries Regulatory Elements Gene Expression Disruption Long Non-coding RNAs Enhancers Embryonic Development Leukemias Lentivirus Packaging HEK293 T Cells Cell Preparation Viral Supernatant Filtration Viral Supernatant Concentration MOLM13 Cells
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