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Biochemistry

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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Article DOI: 10.3791/59385-v • 11:36 min • July 25th, 2019
July 25th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
* These authors contributed equally

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Resumen
Automatic Translation

Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.

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In Vitro Ubiquitination Deubiquitination Assays Nucleosomal Histones Purified Components Mechanisms Mutations Genes Enzymes Human Disease Domains Motifs High Throughput Screen Assays Small Molecule Inhibitors Therapeutics Bacteria Lysis PMSF Antiprotease Cocktail Lysis Buffer A Resuspend Sonication Probe Sonicator Centrifuge GSH Beads Chromatography Column Buffer A Glutathione
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