Aislamiento y cultivo de células endoteliales aórticas primarias de cerdos en miniatura

8.4K visualizaciones

Citado por 6

06:38 min.

14 de agosto de 2019

10.3791/59673-v

14 de agosto de 2019

8.4K visualizaciones

Yanli Zhao¹^,²^,³ , Chengjiang Zhao⁴ , David K.C. Cooper⁵ , Ying Lu² , Kewang Luo⁶ , Huiyun Wang³ , Pengfei Chen³ , Changchun Zeng³ , Shaodong Luan¹ , Lisha Mou² , Hanchao Gao¹^,²^,³

¹Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ²Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, ³Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁴Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁵Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, ⁶People's Hospital of Longhua

Se describe un método enzimático eficaz para el aislamiento de las células endoteliales aórticas porcinas primarias (pAEC) de los cerdos en miniatura. Los pAEC primarios aislados se pueden utilizar para investigar la respuesta inmunitaria y de la coagulación en el xenotrasplante.