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Biochemistry

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JoVE Journal Biochemistry
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
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Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Article DOI: 10.3791/59733-v • 07:07 min • July 19th, 2019
July 19th, 2019 •
*1, *2, 1, 2, 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine
* These authors contributed equally

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Resumen
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Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.

Tags

Protein-protein Interactions On-membrane Digestion Technique Binding Proteins MS Analysis Proteomic Analysis Immunoprecipitants Polyacrylamide Gel Electrophoresis Antibody Conjugation Magnetic Beads Lysate Preparation Centrifugation Magnetic Separation
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