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Panmyeloid differentiering af humant navlestrengsblod afledt CD34+ hæmatopoietisk stamcelle-og progenitorceller

Article DOI: 10.3791/59836-v • 10:25 min • August 9th, 2019
August 9th, 2019 •
1, 1, 1
1Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

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Her præsenterer vi en protokol for immunophenotypic karakterisering og cytokin induceret differentiering af navlestrengsblod afledt CD34+ hæmatopoietisk stilk og stamceller til de fire myeloid lineages. Ansøgningerne i denne protokol omfatter undersøgelser af effekten af myeloid sygdoms mutationer eller små molekyler på myeloid differentiering af CD34+ celler.

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Udviklingsmæssige biologi CD34+ celler myeloid differentiering hæmatopoietisk stilk og stamceller granulocyt monocyt erythrocyt megakaryocyte almindelig myeloid progenitorceller granulocyt monocyt progenitorceller megakaryocyte erythroidprogenitor flow cytometri
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