Cord Blood Isolation
Cord Blood Isolation - Related Videos
14:06
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
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Cited by 2 •
2013
Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.
07:26
Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
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Cited by 16 •
2017
The goal of this protocol is to isolate endothelial progenitor cells from umbilical cord blood. Some of the applications include using these cells as a biomarker for identifying patients with cardiovascular risk, treating ischemic diseases, and creating tissue-engineered vascular and heart valve constructs.
05:23
Isolating Cord Blood Hematopoietic Stem Cells to Generate Human Immune System Mice
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2025
This video demonstrates a technique for generating human immune system (HIS) mice. CD34+ hematopoietic stem cells (HSCs) are isolated from human cord blood buffy coat cells using antibody-coated magnetic beads. The CD34+ HSCs are expanded in a growth medium enriched with cytokines and cryopreserved. Finally, the thawed HSCs are injected into an immunodeficient mouse pup to establish the HIS mouse model.
03:08
Spinal Cord Extraction: A Protocol to Isolate Murine Spinal Cord for In Vitro Studies
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2023
In this video, we describe a step-by-step protocol to isolate spinal cord from a mouse model for in vitro studies.
09:19
Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury
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Cited by 16 •
2015
Contrast Enhanced Ultrasound imaging is a reliable in-vivo tool for quantifying spinal cord blood flow in an experimental rat spinal cord injury model. This paper contains a comprehensive protocol for application of this technique in association with a contusion model of thoracic spinal cord injury.
FAQs
The separation depends on differences in cell density. During centrifugation, cord-blood components migrate to distinct positions within the density gradient, allowing the mononuclear fraction to be recovered apart from plasma, erythrocytes, and other components. This physical partitioning enriches lymphocytes and monocytes for downstream immunology experiments instead of analyzing the whole sample.
Lymphocytes and monocytes belong to the peripheral blood mononuclear cell fraction targeted by the separation. Recovering this population gives investigators access to immune cells for examining neonatal immune development, cellular composition, and responses to infectious agents. Separating them from erythrocytes and other blood components also makes subsequent cellular analyses more focused and comparable.
Sterile processing helps preserve the suitability of cord-blood samples for laboratory analysis and potential clinical use. It is especially relevant when investigators plan to study immune-cell function or evaluate cellular material for transplantation-related research. Maintaining controlled handling conditions supports more reliable comparisons between samples and reduces complications associated with unwanted contamination.
Cord blood contains immune cells that are developmentally distinct, so their composition and function may not represent those of more mature immune-cell populations. This characteristic makes the samples valuable for studying neonatal immunity and immune development. It also means that researchers must interpret cellular responses in the context of early-life biology when examining infection-related outcomes.
A typical workflow begins with collecting blood from the umbilical cord and placenta, followed by processing under sterile conditions. Density-gradient centrifugation then separates the mononuclear cells from plasma, erythrocytes, and other components. The resulting fraction can be recovered for laboratory analysis or for studies requiring selected cord-blood cell populations.
Isolated cord-blood cells allow researchers to examine the cellular composition and function of neonatal immune populations, including responses to infectious agents. Because the preparation enriches mononuclear cells, investigators can focus on lymphocytes and monocytes rather than the complete blood mixture. These findings can help characterize early immune responses and compare results across standardized samples.
The method is useful when studies require access to cord-blood cell populations associated with hematopoietic stem-cell research, immune development, transplantation, or regenerative medicine. Separating the sample into defined cellular fractions supports more targeted evaluation of composition and function. Its clinical relevance comes from examining whether cord-blood-derived material may contribute to transplantation or regenerative applications.