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Immunology and Infection

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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

Article DOI: 10.3791/59973-v • 08:52 min • July 26th, 2019
July 26th, 2019 •
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1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine

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We present a protocol for using a protein microarray constructed by printing influenza antigens onto nitrocellulose-coated slides to simultaneously probe serum for multiple antibody isotypes against over 250 antigens from different virus strains, thus allowing measurement of the breadth of serum antibodies across virus subtypes.

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Influenza Antigen Microarray Serum Antibodies Virus Subtypes Measurement Antibody Isotypes Blood Sample Volume High-throughput Measurement Antigenic Landscape Influenza Strains Influenza Infection Antigen Microarray Technique Pathogens Antibodies Against Antigen Targets Serum Specimens Infectious Disease Cases Healthy Controls In-person Workshops Procedure Microarray Printer Low Volume Microarray Spotting Pins 384-well Source Plate Nitrocellulose Coated Glass Slide
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