Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / A Macrophage Reporter Cell Assay to Examine…
JoVE Journal
Bioengineering

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Bioengineering
A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
Journal (Bioengineering)
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
Journal (Bioengineering)
Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
 

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Article DOI: 10.3791/60317-v • 07:55 min • January 7th, 2020
January 7th, 2020 •
1, 1, 1
1Department of Chemical Engineering, Queen's University

Capítulos

Resumen
Automatic Translation

This protocol provides researchers with a rapid, indirect method of measuring TLR-dependent NF-кB/AP-1 transcription factor activity in a murine macrophage cell line in response to a variety of polymeric surfaces and adsorbed protein layers that model the biomaterial implant microenvironment.

Tags

Macrophage Reporter Cell Assay Toll-like Receptor NF-kB Signaling AP-1 Signaling Adsorbed Protein Layers Polymeric Surfaces Enzymatic Assay PMMA Chloroform Glass Scintillation Vial Magnetic Stir Bar Borosilicate Glass Microscope Slide Spin Coater 70% Ethanol-sprayed Clean Box Eight-chamber Sticky Wells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter