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从一个成年小鼠脑半球分离区域特异性微胶质,进行深度单细胞RNA测序
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
DOI:

09:49 min

December 03, 2019

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Capítulos

  • 00:05Título
  • 00:43Brain Region Dissection
  • 01:43Mechanical Tissue Dissociation
  • 02:40Myelin Removal
  • 04:25Staining for Fluorescence-activated Cell Sorting
  • 05:18Index FACS Sorting
  • 05:46Single-cell RNA-sequencing Library Preparation
  • 07:17Results: Isolation of Region-Specific Microglia for Deep Single-Cell RNA Sequencing
  • 09:16Conclusion

Summary

Traducción Automática

我们为从成年小鼠大脑半球的不同解剖区域分离微胶质提供方案,然后为全长转录体进行深度单细胞RNA测序的半自动库制备。该方法将有助于阐明微胶质在健康和疾病中的功能异质性。

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