Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Isolation of Stem-like Cells from 3-Dimensional…
JoVE Journal
Cancer Research

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Cancer Research
Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
Journal (Cancer Research)
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
Journal (Cancer Research)
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
 

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

Article DOI: 10.3791/60357-v • 09:06 min • December 13th, 2019
December 13th, 2019 •
1, 1, 1, 1, 1,2,3
1Department of Urology, College of Medicine, University of Illinois at Chicago, 2Department of Pathology, College of Medicine, University of Illinois at Chicago, 3University of Illinois Cancer Center, College of Medicine, University of Illinois at Chicago

Capítulos

Resumen
Automatic Translation

Using human primary prostate epithelial cells, we report a novel biomarker-free method of functional characterization of stem-like cells by a spheroid-based label-retention assay. A step-by-step protocol is described for BrdU, CFSE, or Far Red 2D cell labeling; three-dimensional spheroid formation; label-retaining stem-like cell identification by immunocytochemistry; and isolation by FACS.

Tags

Stem-like Cells 3-dimensional Spheroid Cultures Biomarker-free Assay Label-retention Assay FACS CFSE Far Red Labeling Prostate Cancer Cells Cancer Stem Cells Therapeutic Resistance Disease Progression Normal Stem Cell Isolation Epithelial Cell Cancers Breast Cancer Colorectal Cancer Fibronectin Solution Culture Dishes Prostate Epithelial Cell Growth Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter