Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Genetic Modification of Cyanobacteria by…
JoVE Journal
Biology

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Biology
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)
Journal (Biology)
A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Journal (Biology)
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
 

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Article DOI: 10.3791/60451-v • 08:25 min • October 31st, 2019
October 31st, 2019 •
*1,2,3, *1,2, *1,2, 2,3, 1,2
1Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 2Centre for Synthetic and Systems Biology, University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

Here, we present a protocol describing how to i) assemble a self-replicating vector using the CyanoGate modular cloning toolkit, ii) introduce the vector into a cyanobacterial host by conjugation, and iii) characterize transgenic cyanobacteria strains using a plate reader or flow cytometry.

Tags

Genetic Modification Cyanobacteria Conjugation CyanoGate Modular Cloning Toolkit Synthetic Biology Renewable Biotechnology Heterologous DNA Transformable Species Triparental Mating Cyanobacterial Strains E.coli Strains Centrifuge Vortex Bacteria Mix Conjugation Process Level One Assemblies T Acceptor Vector End Link Vector Thermal Cycl
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter