Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Yiding Qi; Xiang Miao; Lian Xu; Mengxia Fu; Shi Peng; Kailei Shi; Jun Li; Maoqing Ye; Ruogu Li

doi:10.3791/60691

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Aislamiento, cultivo e inducción adipogénica de células madre originales adiposas de raíz de la cresta neuronal del tejido adiposorio

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

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Biología del desarrollo

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JoVE Revista Biología del desarrollo

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Aislamiento, cultivo e inducción adipogénica de células madre originales adiposas de raíz de la cresta neuronal del tejido adiposorio

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6,026 Views

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08:31 min

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March 02, 2020

DOI:

10.3791/60691-v

DOI:

10.3791/60691-v

•

08:31 min

March 02, 2020

•

5987 Views

Yiding Qi*¹, Xiang Miao*², Lian Xu³, Mengxia Fu³, Shi Peng⁴, Kailei Shi⁵, Jun Li⁴, Maoqing Ye⁵, Ruogu Li¹

¹Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University ²Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences ³Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine ⁴Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine ⁵Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University

Summary

Presentamos un protocolo para el aislamiento, el cultivo y la inducción adipogénica de células madre derivadas de cresta neural derivadas de la cresta adiposa (NCADSC) del tejido adiposorio periaortico de Wnt-1 Cre^+/-; Ratones Rosa26^RFP/+. Los NCADSC pueden ser una fuente de aXenatos de fácil acceso para modelar adipogénesis o lipogénesis in vitro.