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JoVE Journal Cancer Research
Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells
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Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

Article DOI: 10.3791/61270-v • 08:54 min • May 20th, 2020
May 20th, 2020 •
1, 1, 2, 1
1Department of Radiation Oncology, Arthur G. James Comprehensive Cancer Center and Richard L. Solove Research Institute, The Ohio State University Medical Center, 2Department of Veterinary Biosciences, Comparative Pathology and Digital Imaging Shared Resource, The Ohio State University

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Resumen
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Intravenous injection of cancer cells is often used in metastasis research, but the metastatic tumor burden can be difficult to analyze. Herein, we demonstrate a tail-vein injection model of metastasis and include a novel approach to analyze the resulting metastatic lung tumor burden.

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Pathological Analysis Lung Metastasis Lateral Tail-vein Injection Tumor Cells Digital Image Analysis Quantify Lung Metastases Time And Cost Effective Comprehensive Analysis Human Breast Cancer Cells Cell Culture Medium Cell Concentration Trypsin Centrifugation PBS Injection Procedure Insulin Syringe Needle Bending Mouse Restraint
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