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Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
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Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

Article DOI: 10.3791/61279-v • 11:55 min • June 16th, 2020
June 16th, 2020 •
1, 1, 1, 1, 1,2,3
1Okinawa Institute of Science and Technology Graduate University, 2Francis Crick Institute, 3UCL Genetics Institute, Department of Genetics, Evolution and Environment, University College London

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Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions.

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Streamlined Sampling Cultivation Pelagic Cosmopolitan Larvacean Oikopleura Dioica Culture System Marine Tunicate Baseline Resources Biological Fields Ecology Development Genomics Evolution Model Organism Algal Food Stock Culture Chaetoceros Calcitrans Autoclaved Seawater Vitamins Solution A Sodium Silicate Streptomycin C.calcitrans Inoculant Algal Species Culture Types Incubator Continuous Lighting Long-term Storage
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