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Immunology and Infection

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Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Article DOI: 10.3791/61359-v • 09:00 min • June 24th, 2020
June 24th, 2020 •
*1, *1, 1, 1,2,3
1Department of Microbiology, Immunology, and Cell Biology, West Virginia University, 2Department of Neuroscience, West Virginia University, 3Rockefeller Neuroscience Institute, West Virginia University
* These authors contributed equally

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This protocol describes the use of flow cytometry to identify the changes in immune cell composition, cytokine profile, and chemokine profile in the pulmonary environment following transient middle cerebral artery occlusion, a murine model of ischemic stroke.

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Immune Cells Proinflammatory Mediators Pulmonary Environment Ischemic Stroke Flow Cytometric Analysis Lung Immune Cell Types Cytokines Chemokines Allergic Response Infection Transcardial Perfusion Anesthetized Mouse Surgical Platform Peritoneum Thoracic Cavity Dissection Scissors Diaphragm Rib Cage Right Atrium
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