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Journal / Biochemistry / 实时测量核苷酸与完整功能性膜蛋白的结合…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
实时测量核苷酸与完整功能性膜蛋白的结合
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实时测量核苷酸与完整功能性膜蛋白的结合

Article DOI: 10.3791/61401-v • 08:33 min • March 11th, 2021
March 11th, 2021 •
1, 1, 1,2
1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

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该协议提出了一种在细胞环境中实时测量腺嘌呤核苷酸与受体结合的方法。结合测量为三硝基苯基核苷酸衍生物与用非规范荧光氨基酸标记的蛋白质之间的Förster共振能量转移（FRET）。

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本月在JoVE上，第169期，配体结合，膜蛋白，离子通道，核苷酸，方法，膜片钳，荧光，光谱，受体
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