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Bioengineering

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JoVE Journal Bioengineering
Manipulation de cellules souches neurales et de neurones dans les tranches de cerveau à l’aide de microinjection robotique
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Manipulation de cellules souches neurales et de neurones dans les tranches de cerveau à l’aide de microinjection robotique

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Ce protocole démontre l’utilisation d’une plate-forme robotique pour la microinjection en cellules souches neurales simples et neurones en tranches de cerveau. Cette technique est polyvalente et offre une méthode de suivi des cellules dans les tissus à haute résolution spatiale.

Tags

Bioingénierie numéro 167 développement du cerveau cellules souches traçage de lignée robotique neurone microinjection tranche de tissu organotypique manipulation cellulaire unique
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