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Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

Article DOI: 10.3791/61640-v • 10:10 min • August 19th, 2020
August 19th, 2020 •
1, 1, 2, 3, 1, 4, 4, 1, 1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

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Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.

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Adipose Tissue Structure Methylsalicylate Clearing 3D Imaging Pathological Dysfunction White Adipose Tissue Formaldehyde PBS Ethanol Methyl Salicylate Clearing Process Toxic Solvent Fixative Glycine Triton X-100
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