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Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Article DOI: 10.3791/61871-v • 08:46 min • September 22nd, 2020
September 22nd, 2020 •
1, 2
1Graduate School of Medicine, Ehime University, 2Proteo-Science Center, Ehime University

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This protocol describes an efficient cell-free method for production of high-quality proteoliposome by bilayer-dialysis method using wheat cell-free system and liposomes. This method provides suitable means for functional analysis of membrane proteins, drug targets screening, and antibody development.

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Cell-free Production Proteoliposomes Functional Analysis Antibody Development Membrane Proteins Drug Discovery Biochemical Studies Biophysical Studies Structural Membrane Protein Studies In-vitro Synthesis Purified Proteoliposomes Transcription Reaction Mixture Translation Buffer Small Scale Translation Dialysis Cups Liposomes Lipid Solution Evaporation Flask Rotary Evaporator
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