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Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Article DOI: 10.3791/61956-v • 06:38 min • February 24th, 2021
February 24th, 2021 •
*1, *2, 1, 3, 1, 1, 1, 1, 1
1College of Life Sciences, Nanjing Agricultural University, 2Department of Criminal Science and Technology, Nanjing Forest Police College, 3Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition, Leibniz University Hannover
* These authors contributed equally

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A precise and reproducible method for in vivo nucleosides/nucleotides quantification in plants is described here. This method employs an HPLC-MS/MS.

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Plant Sample Preparation Nucleoside Nucleotide Content Measurement HPLC-MS/MS Metabolites Quantification Sample Pre-treatments LC-MS/MS Analyses Plants Organisms Procedure Changhua Zhu Chen Laboratory Arabidopsis Seeds Sterilized Agar Plates Murashige And Skoog Nutrients Growth Chamber Light/dark Cycle Metabolite Extraction Freeze Plant Tissues Mixer Mill Extraction Solution
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