Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (<em>Oryzias latipes</em>)

Muhammad Rahmad Royan; Shinji Kanda; Daichi Kayo; Weiyi Song; Wei Ge; Finn-Arne Weltzien; Romain Fontaine

doi:10.3791/62006

Procedimientos de gonadectomía y muestreo de sangre en el modelo de teleósto de tamaño pequeño ja...

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Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Procedimientos de gonadectomía y muestreo de sangre en el modelo de teleósto de tamaño pequeño japonés Medaka (Oryzias latipes)

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05:31 min

December 11, 2020

DOI: 10.3791/62006-v

Muhammad Rahmad Royan¹, Shinji Kanda², Daichi Kayo³, Weiyi Song⁴, Wei Ge⁴, Finn-Arne Weltzien¹, Romain Fontaine¹

¹Physiology Unit, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, ²Laboratory of Physiology, Atmosphere and Ocean Research Institute,The University of Tokyo, ³Department of Biological Sciences, Graduate School of Science,The University of Tokyo, ⁴Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences,University of Macau

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Overview

The article presents a quick protocol for gonadectomizing Japanese medaka (Oryzias latipes) and sampling blood to explore the role of sex steroids in animal physiology. This method allows researchers to effectively investigate the physiological functions of gonads and how these relate to neuroendocrine plasticity in teleosts and vertebrates.

Key Study Components

Research Area

Gonadectomy techniques in teleost fish
Investigation of sex steroids' physiological roles
Neuroendocrine system plasticity

Background

Japanese medaka is a widely used model in research
Understanding gonads' functions is critical for studying vertebrate physiology
Previous techniques lacked high survival rates

Methods Used

Protocols for ovariectomy and orchidectomy
Japanese medaka (Oryzias latipes) as the biological model
Blood sampling techniques and hormone analysis

Main Results

100% survival rate in females and 94% in males post-surgery
Significantly lower estrogen (E2) levels in ovariectomized fish compared to sham-operated fish
Reduced 11-ketotestosterone (11-KT) levels in orchidectomized males

Conclusions

This study demonstrates an effective method for gonadectomy with implications for hormone research
Findings highlight the role of sex steroids in the physiology of small teleost models

Frequently Asked Questions

What is the significance of gonadectomy in teleosts?

It allows researchers to study the physiological function of gonads and the impact of sex steroids on various biological processes.

Why is Japanese medaka commonly used in research?

Its small size, ease of handling, and well-characterized genetics make it ideal for laboratory studies.

How does this protocol ensure high survival rates?

Proper training in surgical techniques and careful execution of the procedure contribute to the high survival rate of the fish.

What are the consequences of gonad removal in fish?

Gonad removal alters hormone levels, affecting various physiological functions, including reproductive performance.

Can this method be adapted for other fish species?

While optimized for medaka, aspects of the protocol could be adapted for other small teleost species with proper adjustments.

What are the potential applications of this research?

It may provide insights into fish physiology, evolutionary biology, and signaling pathways related to sex steroids.

Are there any ethical considerations for this procedure?

As with all animal research, proper ethical guidelines must be followed to ensure the welfare of the fish used in experiments.

El artículo describe un protocolo rápido para gonadectomizar y tomar muestras de sangre de los pequeños peces teleóseos, utilizando medaka japonés(Oryzias latipes)como modelo, para investigar el papel de los esteroides sexuales en la fisiología animal.

Este método ayudará a responder preguntas clave sobre la función fisiológica de las gónadas, incluido el papel de los esteroides sexuales en la plasticidad y regulación del sistema neuroendocrino en los teleósteos y, en mayor medida, en los vertebrados. Este protocolo permite realizar la gonadectomía con una alta tasa de éxito en teleósteos de pequeño tamaño, que es ampliamente utilizado en la comunidad investigadora. Para tener éxito y lograr una alta tasa de supervivencia, esta técnica requiere un entrenamiento adecuado, particularmente en la evaluación de las gónadas y la sutura de los peces.

Comience sacando al pez de la solución anestésica y colocándolo horizontalmente de lado bajo un microscopio de disección. Para realizar la ovariectomía en las mujeres, raspe las escamas en el área de la incisión. Realice suavemente una incisión de dos a 2,5 milímetros entre las costillas y las aletas pélvicas y anales con una cuchilla de afeitar.

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