Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Cell Dissociation from the Tongue Epithelium and…
JoVE Journal
Developmental Biology

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Developmental Biology
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
Journal (Developmental Biology)
Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions (Video) | JoVE
Journal (Developmental Biology)
Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions (Video) | JoVE
 

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Article DOI: 10.3791/62163-v • 06:56 min • January 21st, 2021
January 21st, 2021 •
1, 1, 1, 1
1Regenerative Bioscience Center; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia

Capítulos

Resumen
Automatic Translation

We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.

Tags

Cell Dissociation Tongue Epithelium Mesenchyme/connective Tissue Embryonic-day 12.5 8-week-old Mice High Yield Quality Cells Experimental Analysis Single Cell RNA Sequencing Primary Stem Cell Cultures Protocol Mammalian Tongue Euthanized Pregnant Female Mouse Surgical Area 70% Ethanol Operating Site Dissecting Scissors Uterine Horns Embryos Tyrode's Solution Dissecting Microscope Mini Scissors Fine Forceps Mouth Cavity Mandible Sterile Tyrode's Solution Enzyme Mixture Dispase And Collagenous
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter