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Immunology and Infection

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JoVE Journal Immunology and Infection
Aufreinigung und Expansion von mausinvarianten natürlichen Killer-T-Zellen für in vitro und in vivo Studien
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Aufreinigung und Expansion von mausinvarianten natürlichen Killer-T-Zellen für in vitro und in vivo Studien

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Wir beschreiben ein schnelles und robustes Protokoll, um invariante natürliche Killer-T-Zellen (iNKT) aus Mausmilz anzureichern und sie in vitro auf geeignete Zahlen für In-vitro- und In-vivo-Studien zu erweitern.

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Immunologie und Infektion Ausgabe 168 iNKT-Zellen Expansion Maus Vα14 CD1d Anti-CD3/CD28-Kügelchen Aktivierung Lymphozyten
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