Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology,Chinese Academy of Sciences, 2International College,University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai,Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research,Kenya Medical Research Institute
Capítulos
- 00:04Introduction
- 00:48cDNA and Droplet Digital Polymerase Chain Reaction (ddPCR) Preparation
- 02:03Automated Droplet Generation
- 02:59PCR Amplification and Droplet Reading
- 04:09Data Analysis
- 06:01Results: SARS‐CoV‐2 Detection Using a Two‐Color ddPCR System
- 07:07Conclusion
Dette arbejde opsummerer trin til udvikling af forskellige assays til SARS-CoV-2-detektion ved hjælp af et tofarvet ddPCR-system. Trinene er detaljerede, og der er inkluderet noter om, hvordan man forbedrer assays og eksperimentets ydeevne. Disse assays kan bruges til flere SARS-CoV-2 RT-ddPCR-applikationer.
