Establecimiento y manipulación genética de organoides hepatocitos murinos
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February 12, 2022
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Summary
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Se estableció un sistema de cultivo de organoides 3D ex vitro a largo plazo a partir de hepatocitos de ratón. Estos organoides pueden ser atravesados y manipulados genéticamente por infección por lentivirus de construcción shRNA/ectópica, transfección de siRNA e ingeniería CRISPR-Cas9.
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Cite this Article
Lian, J., Meng, X., Zhang, X., Hu, H. Establishment and Genetic Manipulation of Murine Hepatocyte Organoids. J. Vis. Exp. (180), e62438, doi:10.3791/62438 (2022).
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