Aspectos prácticos de la preparación de muestras y la configuración de 1H R1ρ Experimentos de dispersión de relajación de ARN
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July 09, 2021
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Presentamos un protocolo para medir la dinámica de micro a milisegundos en 13C/15N-etiquetado y no etiquetado ARN con 1H R1ρ dispersión de dispersión de relajación espectroscopia de resonancia magnética nuclear (RMN). El enfoque de este protocolo radica en la preparación de muestras de alta pureza y la configuración de experimentos de RMN.
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Feyrer, H., Schlagnitweit, J., Petzold, K. Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA. J. Vis. Exp. (173), e62470, doi:10.3791/62470 (2021).
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