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Bedömning av global DNA Double-Strand End Samband med BrdU-DNA-märkning i kombination med cellcykeldiskrimineringsavbildning
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Bedömning av global DNA Double-Strand End Samband med BrdU-DNA-märkning i kombination med cellcykeldiskrimineringsavbildning

Article DOI: 10.3791/62553-v • 06:44 min • April 28th, 2021
April 28th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion
* These authors contributed equally

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I det nuvarande protokollet visar vi hur man visualiserar DNA dubbelsträngad end samband under S/G2 fas av cellcykeln med hjälp av en immunofluorescensbaserad metod.

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Biologi nummer 170 BrdU-foci Immunofluorescens DNA-end samband homolog rekombination PARP-1 PARP-hämmare PARP-hämmaresistens BMN673
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