Brdu Labeling
Brdu Labeling - Related Videos
12:46
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
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Cited by 6 •
2011
Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.
03:24
BrdU Immunofluorescence Staining: A Technique to Identify Cells in Different Phases of Cell Cycle
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2023
This video describes the protocol for BrdU immunofluorescence staining. The technique allows the identification of cells in different phases of cell cycle. Addition of DNA dyes and antibody labeling allows the detailed analysis of the fate of the S phase cells at later times.
09:14
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Cited by 4 •
2016
In this protocol, we describe a novel BrdU-ChIP-Slot-Western technique to examine proteins and histone modifications associated with newly synthesized or nascent DNA.
06:44
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
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Cited by 10 •
2021
In the present protocol, we demonstrate how to visualize DNA double-strand end resection during S/G2 phase of the cell cycle using an immunofluorescence-based method.
11:23
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
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Cited by 13 •
2012
Tracking subtle changes in the progression and kinetics of cell cycle stages can be accomplished by use of a combination of metabolic labeling of nucleic acids with BrdU and total genomic DNA staining via Propidium Iodide. This method avoids the need of chemical synchronization of cycling cells, thereby preventing the introduction of non-specific DNA damage, which in turn affects cell cycle progression.
FAQs
DNA denaturation is essential because it exposes the incorporated BrdU within newly synthesized DNA, allowing antibodies to bind it. Without this exposure step, the label would not be accessible for antibody-based detection. This mechanistic link connects a chemical mark on DNA to an observable signal in cells or tissue sections.
It identifies cells that were synthesizing DNA during the relevant labeling period, thereby marking S-phase activity. Counting or locating labeled cells can provide a measure of proliferation and show where DNA synthesis occurs. The result is useful for comparing proliferative activity across cell populations, anatomical regions, or biological conditions.
BrdU detection identifies DNA-synthesizing cells, while immunostaining can link that signal to specific cell types. Microscopy then places the labeled cells within their anatomical context. Together, these readouts distinguish where proliferation occurs and which cells contribute to it, rather than reporting only an overall proliferation measurement.
Cells in culture or tissue are exposed to BrdU so it can be incorporated during DNA synthesis. The sample is then processed to denature DNA, antibodies detect the exposed label, and microscopy or immunostaining reveals labeled cells. This sequence converts incorporation into a spatially interpretable signal for measuring or locating proliferation.
Culture experiments can assess DNA synthesis within a defined cell population, whereas tissue studies preserve anatomical location and allow proliferation to be examined in context. The method therefore supports both controlled cell-based measurements and spatial analysis in biological specimens. Choice depends on whether the central question concerns population activity, tissue organization, or both.
Labeled cells can be examined to follow the generation of new cells and their distribution within a developing, regenerating, or diseased system. When paired with microscopy and cell-type markers, the approach connects DNA synthesis with particular locations and populations. This helps researchers investigate cell production and migration as biological processes change over time.