Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Using Next Generation Sequencing to Identify…
JoVE Journal
Genetics

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Genetics
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Journal (Genetics)
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Controllable Ion Channel Expression through Inducible Transient Transfection
Journal (Genetics)
Controllable Ion Channel Expression through Inducible Transient Transfection
 

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Article DOI: 10.3791/62583-v • 06:59 min • March 31st, 2022
March 31st, 2022 •
*1, *2, 1, 1, 2,3
1Division of Biology, Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory, Kansas State University, 3Department of Diagnostic Medicine/Pathobiology, Kansas State University
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

Presented here is sgRNA/CAS9 endonuclease and next-generation sequencing protocol that can be used to identify the mutations associated with double strand break repair near the CD4 promoter.

Tags

Next Generation Sequencing Mutations Repair CAS9-induced Double-strand Break CD4 Promoter Cost-effective Robust Genomic Locus Transfectable Cell Lines Human Keratinocyte Culture Media Growth Supplement Penicillin Streptomycin Trypsin-EDTA FBS-supplemented Media Centrifuge Tube Concentration Of Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter