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Developmental Biology

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JoVE Journal Developmental Biology
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Article DOI: 10.3791/62865-v • 09:56 min • August 31st, 2021
August 31st, 2021 •
1, 2,3, 1, 2,4, 1
1Neurophotonics and Mechanical Systems Biology, Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)

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Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.

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Direct Force Measurements Subcellular Mechanics Confinement Optical Tweezers Mechanical Properties Cell Nucleus Cell Polarization Differentiation Migration Advantage Technique Subcellular Compartments Membrane Perturbation Cortical Cytoskeleton Manipulation Of Beads Nuclear Mechanics Cell Lines Animal Models Direct Force Sensor Alignment Light Momentum Measurements Viscoelastic Medium Single-cell Preparation Sphere Stage Embryos E3 Media Plastic Pasteur Pipette Fluorescent Protein Expression MRNA Injection Forceps Decurionate Embryos Transfer Embryos To Reaction Container
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