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Bioengineering
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JoVE Journal Bioengineering
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
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Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Article DOI: 10.3791/62944-v • 10:51 min • October 13th, 2021
October 13th, 2021 •
*1, *1, 1, 1, 1
1Department of Physiology and Biomedical Engineering, Mayo Clinic
* These authors contributed equally

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This article describes encapsulation of human pluripotent stem cells (hPSCs) using a co-axial flow focusing device. We demonstrate that this microfluidic encapsulation technology enables efficient formation of hPSC spheroids.

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Microfluidic Fabrication Core-shell Microcapsules Human Pluripotent Stem Cells 3D Cultures Encapsulation Strategy Spheroid Formation Cultivation Multi-well Plates Stirred Bioreactors Pestilence Survivability Shear Protection Mechanical Energy Stem Cell Therapy Cellular Product Generation Scalable Efficient Cost-effective Encapsulation Technology Cell Types Core Stock Solution PEG Density Gradient Media DMEM/F-12 Media Syringe Filter Mineral Oil Surfactant
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