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Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy

Article DOI: 10.3791/63188-v • 14:56 min • April 21st, 2023
April 21st, 2023 •
1,2, 1,2, 1, 1, 1, 1,2
1Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, 2The Wohl Institute for Translational Medicine

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We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.

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Cardiac Metabolism Isolated Perfused Mouse Heart Hyperpolarized Pyruvate 13C/31P NMR Spectroscopy MR Studies Protocol Carbon-13 Pyruvate Metabolism Krebs-Hensleit Buffer NMR Spectrometer Cardiac Energetics PH Measurement Adenosine Triphosphate Phosphocreatine Inorganic Phosphate Hyperpolarization Lactate Measurement Real-time Monitoring
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