Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Purification of Endogenous Drosophila Transient…
JoVE Journal
Biochemistry

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Biochemistry
Purification of Endogenous Drosophila Transient Receptor Potential Channels
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
Journal (Biochemistry)
Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
Journal (Biochemistry)
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
 

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

Capítulos

Resumen
Automatic Translation

Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.

Tags

Purification Endogenous Drosophila TRP Channels Protocol Purified Full-length TRP Channels Electrophysiological Properties Structural Information INAD Protein Complex Nickel Beads Fly Heads Yuyang Liu Technician PGEX 4T-1 TRP 1261-1265 Plasmid E.coli BL21 Cells Calcium Chloride Heat Shock Transformation Method Luria Bertani Medium Seeding Culture Optical Density Isopropyl-beta-D-1-thiogalactopyranoside Overexpression Centrifugation Phosphate-buffered Saline High Pressure Homogenizer Circularly Pass
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter