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Biochemistry

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A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Article DOI: 10.3791/63387-v • 13:43 min • January 31st, 2022
January 31st, 2022 •
1, 1,2
1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University

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This article describes how to effectively utilize three cryo-EM processing platforms, i.e., cryoSPARC v3, RELION-3, and Scipion 3, to create a single and robust workflow applicable to a variety of single-particle data sets for high-resolution structure determination.

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Robust Single-Particle Cryo-Electron Microscopy Cryo-EM CryoSPARC RELION Scipion Image Processing Structure Calculations 3D Maps Algorithms Combination Of Programs Workflow CryoSPARC V3 Web Browser Workspace Job Builder Import Movies Gain Reference File Path Acquisition Parameters Queue Patch Motion Correction Patch CTF Estimation Micrographs
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