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In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice.

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In Vivo Imaging Biological Tissues Two-photon Fluorescence Stimulated Raman Scattering Microscopy Subcellular Resolution Chemical Specificity Cellular Metabolism Immune Response Tissue Remodeling Mouse Spinal Cord Neurodegenerative Diseases Spinal Cord Injury Titanium Sapphire Laser OPO Control Panel Modulation Depth Stokes Beam High-speed Photo Detector Oscilloscope
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