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Bioengineering

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JoVE Journal Bioengineering
Storstilet, automatiseret produktion af fedtafledte stamcellesfæroider til 3D-bioprint
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Storstilet, automatiseret produktion af fedtafledte stamcellesfæroider til 3D-bioprint

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Her beskriver vi den store produktion af fedtafledte stromale / stamcelle (ASC) sfæroider ved hjælp af et automatiseret pipetteringssystem til at frø cellesuspensionen og dermed sikre homogenitet af sfæroid størrelse og form. Disse ASC-sfæroider kan bruges som byggesten til 3D-bioprinting-tilgange.

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