Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:04Introduction
- 01:01Preparation and Sample Collection
- 03:03Sample Processing for Electron Microscopy
- 04:25Imaging and Measuring Cell Shape, Size, and Microridge Pattern
- 06:36Results: Imaging Analysis Using Scanning Electron Microscopy
- 07:28Conclusion
This protocol focuses on damaging the ocular surface of zebrafish through abrasion to assess the subsequent wound closure at the cellular level. This approach exploits an ocular burr to partly remove the corneal epithelium and uses scanning electron microscopy to track changes in cell morphology during wound closure.
