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JoVE Journal Bioengineering
Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint
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Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint

Article DOI: 10.3791/63617-v • 12:06 min • August 2nd, 2022
August 2nd, 2022 •
*1,2, *1,2, 1,3, 1,2
1Department of Biological Sciences and Bioengineering, Indian Institute of Technology-Kanpur, 2The Mehta Family Centre for Engineering in Medicine, Indian Institute of Technology-Kanpur, 3Mechanobiology Institute, National University of Singapore
* These authors contributed equally

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Infrapatellar fat pad mesenchymal stem cells (IFP-MSCs) can be isolated easily from the infrapatellar fat pad of the knee joint. They proliferate well in vitro, form CFU-F colonies, and differentiate into adipogenic, chondrogenic, and osteogenic lineages. Herein, the methodology for the isolation, expansion, and differentiation of IFP-MSCs from goat stifle joint is provided.

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Isolation Expansion Differentiation Mesenchymal Stem Cells Infrapatellar Fat Pad Goat Stifle Joint IFP-MSCs Stem Cell Type Protocol Reliable Method Reproducible Method High-quality IFP-MSCs Multiple Lineages Regenerative Potential Cartilage Defects Osteoarthritis Cell-based Therapy Tissue Engineering Regenerative Medicine Dr. Sugata Hazra Mr. Aman Mahajan Femorotibial Joints Hind Limbs Sterilized Collection Sample Box Transportation Laboratory Processing 150-millimeter Petri Dish Aseptic Process
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