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Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Article DOI: 10.3791/63629-v • 10:07 min • March 16th, 2022
March 16th, 2022 •
*1, *2,3, 2,3, 1, 2,3, 2,3, 2, 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University
* These authors contributed equally

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This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

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CRISPR/Cas9 Homozygous Mutants Migratory Locust Gene Editing Egg Collection Mutant Screening Homozygous Maintaining Injection Needles Cas9 Protein Single Guide RNA Freshly Laid Egg Pods Microinjection Parameters
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