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Etablering av humane lungeorganoider og proksimal differensiering for å generere modne luftveisorganoider

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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Protokollen presenterer en metode for å utlede humane lungeorganoider fra primære lungevev, utvide lungeorganoidene og indusere proksimal differensiering for å generere 3D- og 2D-luftveisorganoider som trofast fenokopierer det menneskelige luftveisepitelet.

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Biologi utgave 181
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