Airway Epithelium
Airway Epithelium - Related Videos
07:40
Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium
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2025
Air-liquid interface culture is commonly used to develop pseudostratified airway epithelium by differentiating primary normal human bronchial epithelial cells that mimic the apical side of the lung airway. Here, we describe an easy protocol for determining its quality by monitoring its biophysical properties, such as ciliary function and membrane integrity.
13:00
Studying Wnt Signaling During Patterning of Conducting Airways
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Cited by 4 •
2016
The use of reporter mice coupled to whole mount and section staining, microscopy and in vivo assays facilitates the analysis of mechanisms underlying the normal patterning of the respiratory tract. Here we describe how these techniques contributed to the analysis of Wnt signaling during tracheal development.
09:26
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
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Cited by 7 •
2015
Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.
09:28
COVID-19 / Coronavirus Outbreak: Protecting The Airway - Endotracheal Intubation
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2020
In pandemic times, medical staff is becoming a key resource in fighting the infection. To achieve the best medical care, relevant techniques and procedures have to be taught to medical staff to reduce the risk of infection. COVID patients often need mechanical ventilation due to progredient respiratory insufficiency, so an endotracheal intubation becomes a critical procedure in managing these patients. This procedure has an increased risk of infection due to aerosol formation and working with...
03:38
Isolation of Circumvallate Papillae (CVP) Epithelium from Mouse Model: An Enzymatic Procedure to Separate CVP Epithelium from Mouse Tongue
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2025
In this video, we describe the isolation of circumvallate papillae (CVP) epithelium from a murine tongue.
FAQs
Tight junctions between neighboring epithelial cells limit passage through the spaces between cells. In the airway, this supports the barrier that separates inhaled material from underlying tissue. Studying junctional integrity therefore helps researchers assess how injury or disease may compromise protection and permit unwanted interactions.
Ciliary beating works with mucus production as a coordinated clearance system. Goblet cells supply mucus that traps particles and pathogens, while repeated ciliary movement carries that material toward the throat. This relationship is important when evaluating airway defense, because impaired coordination could reduce removal efficiency.
Basal cells provide a renewing population after epithelial injury. Their support matters because damage can affect several protective features at once, including cellular coverage and the coordinated clearance surface described in the airway lining. In medical research, examining repair helps connect epithelial injury with inflammatory or chronic respiratory conditions.
Medical studies examine airway epithelium across asthma, chronic obstructive pulmonary disease, cystic fibrosis, infection, and inflammatory responses. This focus links epithelial barrier and clearance functions with clinically relevant respiratory disorders. Comparing these settings can clarify how different diseases affect the same protective interface.
Cultured airway cells and organoid models provide experimental systems for studying airway epithelial responses. They support evaluation of respiratory toxicants, drug delivery, and emerging therapies, extending investigation beyond descriptions of disease alone. By focusing on epithelial cells in a model system, researchers can examine how these applications relate to airway structure and function.
Airway epithelial models support research on respiratory toxicants, drug delivery, and emerging therapies. These applications use the epithelial interface as a relevant setting for investigating respiratory biology and disease. The models also complement studies of asthma, chronic obstructive pulmonary disease, cystic fibrosis, infection, and inflammatory responses by providing a cellular context for evaluation.