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Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction

Article DOI: 10.3791/63742-v • 05:33 min • July 26th, 2022
July 26th, 2022 •
1, 2
1Electron Microscopy Laboratory, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Science and Technology

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The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.

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Liquid-soft Matter Interaction In Situ Transmission Electron Microscopy Light-induced Observation Bacteria Encapsulation Photosensitizer Sample Preparation TEM Grids Optical Fiber Objective Lens Condenser Lens Amorphous Carbon Microliters Of Bacteria Solution Optical Density Blotting Liquid Spreading Liquid Photosensitizer Application Thin Layer Of Liquid Substrate
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