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Label-Retention Expansion Microscopy (LR-ExM) Enables Super-Resolution Imaging and High-Efficiency Labeling

Article DOI: 10.3791/63793-v • 07:44 min • October 11th, 2022
October 11th, 2022 •
1, 2, 1,2,3
1Center for Complex Biological Systems, University of California Irvine, 2Department of Developmental and Cell Biology, University of California Irvine, 3Department of Chemistry, University of California Irvine

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A protocol of label retention expansion microscopy (LR-ExM) is demonstrated. LR-ExM uses a novel set of trifunctional anchors, which provides better labeling efficiency compared to previously introduced expansion microscopies.

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Label-Retention Expansion Microscopy LR-ExM Super-resolution Imaging High-efficiency Labeling Expansion Microscopy Fluorescence Polymerization Digestion Tri Functional Anchors Signal To Noise Ratio Versatile Method Fluorescent Microscopy Robust Enhanced Signal Culture Cells Removable Chambered Cover Glass Complete Medium Fix Cells PFA PEM Buffer Permeabilization Buffer Streptavidin Solution Cell Blocking Buffer Biotin Solution
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